Dr. Noha G. Bader El Din, Dr. Sally Farouk

Conference 2024 Live Talk

Talk Title

Overexpression of miRNA-374 in Colorectal Cancer Patients and its Impact on Elevated TGFß1 Levels

Authors and Affiliations

Noha G. Bader El Din

Abstract

Background

Background: Colorectal cancer (CRC) is a complex and heterogeneous disease with molecular alterations playing a pivotal role in its initiation and progression. MicroRNAs (miRNAs) are known to be dysregulated in various cancers, including CRC. Among these, miRNA-374 has been implicated in cancer pathogenesis, although its specific role and impact on CRC remain underexplored.

Methods

Methods: Serum samples were collected from 150 Subjects (100 CRC patients and 50 Controls). The expression levels of miRNA-374 and TGFB1 were assessed using quantitative real-time polymerase chain reaction (qRT-PCR) and ELISA Technique, respectively. Statistical analyses were employed to determine correlations and associations between miRNA-374 expression and TGFB1 levels.

Results

Results: Our findings reveal a significant overexpression of miRNA-374 in colorectal cancer patients compared to healthy controls. Moreover, a positive correlation was observed between miRNA-374 expression and elevated TGFB1 levels in CRC patients.

Conclusions

Conclusion: this is the first-time detection of miRNA-374 overexpressed in Egyptian CRC patients. The results suggest a potential regulatory role of miRNA-374 in modulating TGFB1 expression in colorectal cancer and these can be a diagnostic and prognostic CRC biomarker. However, further investigations into the mechanistic aspects of this interaction may contribute to a better understanding of the molecular pathways involved in CRC progression and provide novel insights for targeted therapeutic interventions and contribute to the development of personalized treatment approaches for Egyptian CRC patients.